The Minutemen were a group of seven hired enforcers who conducted operations for a secret society known only as the Trust. They were originally formed in the late 16th century as bodyguards for thirteen aristocratic Trust families who had abandoned Europe in favor of establishing ties in the New World. When England sought to infringe upon the Trust's enterprises by colonizing Roanoke Island, the Minutemen responded, slaughtering the new European aggressors.

The thirteen families that make up The Trust are as follows (the issue numbers in parenthesis reference the issue in which the House was removed, through extermination):

• House of Carlito: Fulvio Carlito (100 Bullets #65)
• House of D'Arcy: Joan D'Arcy (100 Bullets #100)
• House of Dietrich: Megan Dietrich (100 Bullets #100)
• House of Kotias: Helena Kotias (100 Bullets #86)
• House of Madrid: Anwar Madrid, Rose Madrid (100 Bullets #56)
• House of Medici: Augustus Medici, Benito Medici (100 Bullets #100)
• House of Nagel: Axel Nagel, Anna Nagel, Lars Nagel (100 Bullets #74)
• House of Peres: Daniel Peres (100 Bullets #25)
• House of Rhone: Sigmar Rhone (100 Bullets #84)
• House of Simone: Mia Simone (100 Bullets #76)
• House of Vasco: Javier Vasco (100 Bullets #98)
• House of Vermeer: Tibo Vermeer (100 Bullets #100)
• House of Von Hagen: Constance Von Hagen (100 Bullets #86)

There is also a fourteenth House, elected in the final moments of The Trust's existence, with their new pact to no longer absorb any more of the destroyed Houses, it is voted that something must be done with the House of Vasco, so it becomes the House of Graves and Philip is made a member of The Trust, electing Dizzy to replace him as Agent. It is his move against another House of the Trust (Medici) that leads to Dizzy being required to kill him, and thus the series closes.